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monoclonal mouse anti human pcna  (Bio-Rad)
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Bio-Rad monoclonal mouse anti human pcna
Fig. 1. Time course changes of renal function (A), fibrosis (B and C), apoptosis (D), proliferating cell nuclear antigen <t>(PCNA;</t> E) and ED1 (F) in the kidneys of rats submitted to nephrotoxic nephritis (NTN). Data are mean SEM. Statistical significance when compared to respective controls is *P 0.05 and **P 0.01; N 6 to 8 in NTN, N 4 in controls. Abbreviations are: UP, proteinuria; SCr, serum creatinine; G, glo- merulus; T, tubules; I, interstitium. The score of apoptosis, PCNA or ED1 is the number of in situ end-labeling (ISEL) and PCNA nuclei or ED1 cells per glomerulus, per 400 tubular cells or per 400 interstitial field.
Monoclonal Mouse Anti Human Pcna, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ihc+nuclear+image+analysis+tool/pm12234300-86-33-42?v=Bio-Rad
Average 93 stars, based on 1 article reviews
monoclonal mouse anti human pcna - by Bioz Stars, 2026-08
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Fig. 1. Time course changes of renal function (A), fibrosis (B and C), apoptosis (D), proliferating cell nuclear antigen (PCNA; E) and ED1 (F) in the kidneys of rats submitted to nephrotoxic nephritis (NTN). Data are mean SEM. Statistical significance when compared to respective controls is *P 0.05 and **P 0.01; N 6 to 8 in NTN, N 4 in controls. Abbreviations are: UP, proteinuria; SCr, serum creatinine; G, glo- merulus; T, tubules; I, interstitium. The score of apoptosis, PCNA or ED1 is the number of in situ end-labeling (ISEL) and PCNA nuclei or ED1 cells per glomerulus, per 400 tubular cells or per 400 interstitial field.

Journal: Kidney international

Article Title: A shift in the Bax/Bcl-2 balance may activate caspase-3 and modulate apoptosis in experimental glomerulonephritis.

doi: 10.1111/j.1523-1755.2002.kid587.x

Figure Lengend Snippet: Fig. 1. Time course changes of renal function (A), fibrosis (B and C), apoptosis (D), proliferating cell nuclear antigen (PCNA; E) and ED1 (F) in the kidneys of rats submitted to nephrotoxic nephritis (NTN). Data are mean SEM. Statistical significance when compared to respective controls is *P 0.05 and **P 0.01; N 6 to 8 in NTN, N 4 in controls. Abbreviations are: UP, proteinuria; SCr, serum creatinine; G, glo- merulus; T, tubules; I, interstitium. The score of apoptosis, PCNA or ED1 is the number of in situ end-labeling (ISEL) and PCNA nuclei or ED1 cells per glomerulus, per 400 tubular cells or per 400 interstitial field.

Article Snippet: Polyclonal rabbit anti-mouse Bax (P-19; Santa Cruz Biochemicals), monoclonal mousewere quantitatively analyzed by scanning volume density using a Bio-Rad GS-690 densitometer and Molecular anti-rat Bcl-2 (to confirm Bcl-2 staining; Santa Cruz Bio- chemicals), monoclonal mouse anti-human PCNA (cloneAnalyst version 4 analysis software (Bio-Rad Laboratories Ltd., Hertz, UK).

Techniques: In Situ, End Labeling

Fig. 5. Distribution of Bax and Bcl-2 in control and NTN kidneys. Bax immunostaining was visible in the distal tubules and cortical collecting ducts of control kidneys (200, A) and in the glomeruli (200, B and C), dilated proximal tubules and interstitial cells of NTN kidneys (100, C). Some cells stained for Bax had sloughed into the tubular lumen (400, D). There was no Bcl-2 immunostaining in the control kidneys (200, E) but was present in the atrophied tubules of NTN kidneys (100, F). In the NTN kidneys, double staining of Bax (bright pink) and ISEL (brown) was noted in the atrophied tubules (200, G). Double staining of Bcl-2 (brown) and Bax (bright pink) was also found in atrophied and dilated tubules (400, H) as well as Bcl-2 (bright pink) and PCNA (brown) double staining in the atrophied tubules (400, I). The dark pink and brown pattern indicates composite staining.

Journal: Kidney international

Article Title: A shift in the Bax/Bcl-2 balance may activate caspase-3 and modulate apoptosis in experimental glomerulonephritis.

doi: 10.1111/j.1523-1755.2002.kid587.x

Figure Lengend Snippet: Fig. 5. Distribution of Bax and Bcl-2 in control and NTN kidneys. Bax immunostaining was visible in the distal tubules and cortical collecting ducts of control kidneys (200, A) and in the glomeruli (200, B and C), dilated proximal tubules and interstitial cells of NTN kidneys (100, C). Some cells stained for Bax had sloughed into the tubular lumen (400, D). There was no Bcl-2 immunostaining in the control kidneys (200, E) but was present in the atrophied tubules of NTN kidneys (100, F). In the NTN kidneys, double staining of Bax (bright pink) and ISEL (brown) was noted in the atrophied tubules (200, G). Double staining of Bcl-2 (brown) and Bax (bright pink) was also found in atrophied and dilated tubules (400, H) as well as Bcl-2 (bright pink) and PCNA (brown) double staining in the atrophied tubules (400, I). The dark pink and brown pattern indicates composite staining.

Article Snippet: Polyclonal rabbit anti-mouse Bax (P-19; Santa Cruz Biochemicals), monoclonal mousewere quantitatively analyzed by scanning volume density using a Bio-Rad GS-690 densitometer and Molecular anti-rat Bcl-2 (to confirm Bcl-2 staining; Santa Cruz Bio- chemicals), monoclonal mouse anti-human PCNA (cloneAnalyst version 4 analysis software (Bio-Rad Laboratories Ltd., Hertz, UK).

Techniques: Control, Immunostaining, Staining, Double Staining

Fig. 6. Renal cell apoptosis, proliferation and inflammation. The determination of apoptotic cells using ISEL at 400 magnification in glomerulus (A), tubule (B) and interstitium (C) of NTN kidneys. PCNA staining nuclei at 200 magnification in inflamed glomerulus (D), dilated tubules (E) and expended interstitium (E) of NTN kidneys. ED1 positive cells at 100 magnification in glomeruli and interstitium (F). Double staining for caspase-3 (pink) and ISEL (brown) in the tubule (G, I) and interstitium (H, I) in NTN kidneys.

Journal: Kidney international

Article Title: A shift in the Bax/Bcl-2 balance may activate caspase-3 and modulate apoptosis in experimental glomerulonephritis.

doi: 10.1111/j.1523-1755.2002.kid587.x

Figure Lengend Snippet: Fig. 6. Renal cell apoptosis, proliferation and inflammation. The determination of apoptotic cells using ISEL at 400 magnification in glomerulus (A), tubule (B) and interstitium (C) of NTN kidneys. PCNA staining nuclei at 200 magnification in inflamed glomerulus (D), dilated tubules (E) and expended interstitium (E) of NTN kidneys. ED1 positive cells at 100 magnification in glomeruli and interstitium (F). Double staining for caspase-3 (pink) and ISEL (brown) in the tubule (G, I) and interstitium (H, I) in NTN kidneys.

Article Snippet: Polyclonal rabbit anti-mouse Bax (P-19; Santa Cruz Biochemicals), monoclonal mousewere quantitatively analyzed by scanning volume density using a Bio-Rad GS-690 densitometer and Molecular anti-rat Bcl-2 (to confirm Bcl-2 staining; Santa Cruz Bio- chemicals), monoclonal mouse anti-human PCNA (cloneAnalyst version 4 analysis software (Bio-Rad Laboratories Ltd., Hertz, UK).

Techniques: Staining, Double Staining